Diagnosis of severe sepsis using phospholipids enzymatic assay based on cyclic voltammetry

Abstract

In this work phospholipid quantification was carried out using an enzymatic assay based on cyclic voltammetry of the condensation product of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) and 4-aminoantipyrine (4-AP) with a graphite electrode. For the optimization of electrochemical measurement for the product, electrochemical properties such as the electrochemical window, double layer capacitance (Cdl) and electron transfer rate (kapp) were analyzed for a graphite-electrode and Au-electrode. The phospholipid enzymatic assay based the on electrochemical measurement using the graphite electrode was applied to the diagnosis of sepsis for sera from healthy volunteers (n = 16), patients with systemic inflammatory response syndrome (SIRS, n = 16) and severe sepsis patients (n = 24). Finally, the phospholipid quantification results from the electrochemical measurement were statistically compared with the conventional method based on optical density measurement.

paper information

Enzyme and Microbial Technology  Volume 144, March 2021, 109728

Received 7 July 2020, Revised 12 December 2020, Accepted 14 December 2020, Available online 16 December 2020.

Authors: Jun-Hee Park, Jong-Min Park, Ga-Yeon Lee, Zhiquan Song, Jeong Soo Sung, Min-Jung Kang, Nam Su Ku, Eun Hye Lee, Kwang Hwan Park, Moo Suk Park, Sang-Guk Lee, Jae-Chul Pyun

DOI : https://doi.org/10.1016/j.enzmictec.2020.109728