- Title
- [2021] [Jae-Chul Pyun ] Competitive Immunoassay of SARS‑CoV‑2 Using Pig Sera‑Derived Anti‑SARS‑CoV‑2 Antibodies
- Date
- 2021.09.28
- Writer
- AMRC
- 게시글 내용
-
Competitive immunoassay of SARS-CoV-2 using pig sera-derived anti-SARS-CoV-2 antibodies
Abstract
Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.
paper information
BioChip Journal 2021, vol.15,no.1, pp. 100-108 (9 pages)
- Published:
Authors: Ji-Hong Bong, KIM TAEHOON, JUNG JAE YONG, Soo Jeong Lee, SUNG JEONGSOO, LEE CHANG KYU, Min-Jung Kang, hyun ok kim , JAE-CHUL PYUN