- Title
- [2021] [Jae‑Chul Pyun]Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity
- Date
- 2022.11.21
- Writer
- AMRC
- 게시글 내용
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Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity
Abstract
Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration of anti-spike protein (SP) IgG antibodies using magnetic beads with immobilized human SARS-CoV SP. The enhanced specificity of the purified antibodies to the NP of SARS-CoV-2 was demonstrated using an immunoassay with anti-NP IgG antibodies after the isolation and filtration steps. The binding constants (Kd) of the purified anti-NP IgG antibodies to the NP of SARS-CoV-2 and the SP of SARS-CoV were estimated using a surface plasmon resonance biosensor (SPR). A competitive assay using the two-step purified anti-NP IgG antibodies from pig serum demonstrated (a) the detection of SARS-CoV-2 in viral fluid and (b) the discrimination of SARS-CoV-2 from SARS-CoV, MERS-CoV, and CoV strain 229E in viral fluids.
paper information
BioChip Journal (2021) 15:195–203
Received: 1 February 2021 / Revised: 1 February 2021 / Accepted: 11 March 2021 / Published online: 25 March 2021
Authors: Jaeyong Jung · Ji‑Hong Bong · Hong‑Rae Kim · Jun‑Hee Park · Chang Kyu Lee · Min‑Jung Kang · Hyun Ok Kim ·Jae‑Chul Pyun